August 11, 2020

New breakthrough in nanobiosensor research

Promoted a new type of biosensor. Because of the sub-micron-sized transducers, probes or nano-microsystems, various performances of biosensors have been greatly improved.

Nanotechnology and biotechnology are the two leading technologies in the 21st century, and there are many technical intersections between the two. Among them, nano biosensing technology is expected to become an emerging industry. It is a research field formed by the interpenetration of various disciplines such as biology, chemistry, medicine, physics, and electronic technology. Nano biosensors have the characteristics of high selectivity, fast analysis speed, easy operation, and low price of instruments. They can also be used for online or even in vivo analysis, and have been highly valued and widely used in clinical diagnosis, environmental monitoring, and food industry. In previous studies, the introduction of nanotechnology in the field of biosensors has improved the sensitivity and other performance of biosensors, and promoted new types of biosensors. Because of the sub-micron-sized transducers, probes or nano-microsystems, various performances of biosensors have been greatly improved.

The research team of Zhao Yongsheng, a key laboratory of the Institute of Chemistry, Chinese Academy of Sciences, prepared a more advanced and reusable electrochemiluminescent nanobiosensor. As a valuable detection device, electrochemiluminescent nanobiosensors have received more and more attention in detection applications. Electrochemiluminescence has high stability and low background signal, therefore, electrochemiluminescence has aroused great interest of scientists. At the same time, the regenerative electrochemical luminescence sensor has been extensively studied, because this regenerative sensor can not only reduce the consumption of reaction reagents, but also simplify the experimental design. The nanomaterials that are electrochemically oxidized and reduced can react with the co-reactant on the electrode surface, thereby generating electrochemiluminescence. The researchers of this research group introduced ruthenium bipyridine (Ru (bpy) 32+) nanowires as luminescent probes to modify the electrodes in the electrochemiluminescence sensing system, and effectively enhanced the electrochemical luminescence by reducing graphene oxide (RGO) to achieve The efficient and sensitive detection of biomolecule dopamine. The study also proved that one-dimensional nanomaterials with high specific surface area can be used to prepare electrochemiluminescence sensors, which makes it possible to make the sensors more sensitive, smaller in size, faster in response, and the demand for the samples to be tested. less. After this, the research team prepared an organic core / shell nanostructured nanobiosensor. The researchers used 9,10-diphenylethynylanthracene (BPEA) single crystal nanowires as the core layer, and the peroxalate derivative CPPO sensitive to H2O2 as the shell layer. Chemiluminescence experiments proved that the shell layer was against H2O2. The gas has an ultra-sensitive and highly selective response. On this basis, the scientists also used the evanescent wave coupling between the core and shell to effectively amplify the chemical reaction between CPPO and H2O2 gas, and constructed a BPEA @ CPPO optical waveguide sensor, thereby achieving fast, highly sensitive, Highly selective in situ detection. This study further highlights that the use of high-specific surface area one-dimensional nanomaterials to prepare biosensors can improve the sensitivity of the sensor.

Researchers in the laboratory emphasized that these results provide important theoretical and experimental basis for the research of low-dimensional nanomaterials for the preparation of biosensors. Next, they will use one-dimensional nanomaterials to build nanophotonic student sensor related devices to achieve the perfect combination of nanomaterials, photonics and biology.

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